High School

Which techniques are used in yeast that are not available for other eukaryotic model organisms?

A. Yeast artificial chromosomes
B. RNA interference
C. YEp vector cloning
D. Quelling
E. Chemical mutagenesis

Answer :

Yeast has been an immensely valuable model organism in research due to the vast array of techniques available to researchers.

Some of the techniques used in yeast that are not available for other eukaryotic model organisms are yeast artificial chromosomes (YACs), RNA interference (RNAi), YEp vector cloning and quelling chemical mutagenesis.

YACs are large plasmids that are used to clone large pieces of DNA, such as entire genes. RNAi is a technique that uses small interfering RNAs to silence gene expression, allowing researchers to better understand the function of a gene.

YEp vector cloning is a method of cloning used to clone large fragments of DNA, while quelling chemical mutagenesis uses the chemical quinacrine to induce point mutations in certain regions of the yeast genome. These techniques give researchers the ability to study and manipulate yeast genomes with precision and accuracy, making yeast an indispensable model organism.

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